DNA
Part:BBa_K5412002
Designed by: Divakar Kalivarathan Group: iGEM24_SVCE-CHENNAI (2024-10-02)
CFTR mutant
Design considerations for this sequence include selecting a strong, constitutive promoter for efficient CFTR gene expression, optimizing codon usage for target organisms, ensuring accurate regulatory elements for proper transcription, and incorporating necessary enhancers. Careful sequence validation is crucial to prevent mutations, ensuring the gene's function for Cystic Fibrosis research and therapeutic development.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal EcoRI site found at 486
Illegal EcoRI site found at 2903
Illegal SpeI site found at 2681 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 486
Illegal EcoRI site found at 2903
Illegal SpeI site found at 2681 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 486
Illegal EcoRI site found at 2903 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal EcoRI site found at 486
Illegal EcoRI site found at 2903
Illegal SpeI site found at 2681 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal EcoRI site found at 486
Illegal EcoRI site found at 2903
Illegal SpeI site found at 2681 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2705
REFERENCES:
https://www.cell.com/molecular-therapy-family/methods/fulltext/S2329-0501(23)00120-1
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Categories
Parameters
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